Part:BBa_K2148008

mVenus C.reinhardtii-CO Yellow FP

This is a coding DNA sequence (CDS) for a yellow fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.

Usage and Biology

This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a yellow Venus fluorescent protein for Chlamydomonas reinhardtii.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 668
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 668
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 668
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 668
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 668
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

Rasala, B. A., Barrera, D. J., Ng, J., Plucinak, T. M., Rosenberg, J. N., Weeks, D. P., Oyler, G. A., Peterson, T. C., Haerizadeh, F. and Mayfield, S. P. (2013), Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J, 74: 545–556. doi:10.1111/tpj.12165